Rabbit anti-SORD Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-465A-T maps to a region between residue 1 and 50 of human Sorbitol Dehydrogenase using the numbering given in entry NP_003095.2 (GeneID 6652).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SORD immobilized on solid support.
The epitope recognized by A303-465A maps to a region between residue 1 and 50 of human Sorbitol Dehydrogenase using the numbering given in entry NP_003095.2 (GeneID 6652).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Sorbitol dehydrogenase (SORD) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase, makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor [taken from NCBI Entrez Gene (Gene ID: 6652)].
Alternate Names
epididymis secretory sperm binding protein Li 95n; HEL-S-95n; L-iditol 2-dehydrogenase; sorbitol dehydrogenase; SORD1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.