Rabbit anti-SOX2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-740A maps to a region between residue 250 and 300 of human SRY (sex determining region Y)-box 2 using the numbering given in entry NP_003097.1 (GeneID 6657).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SOX2 immobilized on solid support.
The epitope recognized by A301-740A-T maps to a region between residue 250 and 300 of human SRY (sex determining region Y)-box 2 using the numbering given in entry NP_003097.1 (GeneID 6657).
Additional Product Information
SRY (sex determining region Y)- box 2 (SOX2) is a transcription factor involved in embryonic development and is critical to embryonic stem cell pluripotency. Mutations in the SOX2 gene are associated with microphthalmia syndromic type 3 (MCOPS3) a condition characterized by microphthalmia or anophthalmia.
Alternate Names
ANOP3; MCOPS3; sex determining region Y-box 2; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.