Rabbit anti-SPF45 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-498A maps to a region between residue 351 and 401 of human splicing factor (45kD) using the numbering given in entry NP_116294.1 (GeneID 84991).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SPF45 immobilized on solid support.
The epitope recognized by A302-498A-T maps to a region between residue 351 and 401 of human splicing factor (45kD) using the numbering given in entry NP_116294.1 (GeneID 84991).
Additional Product Information
Splicing factor 45 (SPF45) is an RNA splicing factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. SPF45 is involved in the regulation of alternative splicing and the utilization of cryptic splice sites. It has been shown to promote the utilization of a cryptic splice site created by the beta-110 mutation in the human beta-thalassemia gene. The resulting frameshift leads to sickle cell anemia [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96125].
Alternate Names
45 kDa-splicing factor; RNA-binding motif protein 17; SPF45; splicing factor 45; splicing factor 45kDa
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.