Rabbit anti-SPIN90 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-647A maps to a region between residue 672 and 722 of human SH3 protein interacting with Nck, 90 kDa using the numbering given in entry NP_057537.1 (GeneID 51517).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SPIN90 immobilized on solid support.
The epitope recognized by A302-647A-T maps to a region between residue 672 and 722 of human SH3 protein interacting with Nck, 90 kDa using the numbering given in entry NP_057537.1 (GeneID 51517).
Additional Product Information
SPIN90 plays a role in signal transduction, and may function in the maintenance of sarcomeres and in the assembly of myofibrils into sarcomeres. It also plays an important role in stress fiber formation. The gene encoding SPIN90, NCKIPSD, is involved in therapy-related leukemia by a chromosomal translocation t(3;11)(p21;q23) that involves NCKIPSD and the myeloid/lymphoid leukemia gene [taken from NCBI Entrez Gene (GeneID: 51517)].
Alternate Names
54 kDa VacA-interacting protein; 54 kDa vimentin-interacting protein; 90 kDa SH3 protein interacting with Nck; AF3P21; dia interacting protein; dia-interacting protein 1; diaphanous protein interacting protein; Diaphanous protein-interacting protein; DIP; DIP1; DIP-1; NCK-interacting protein with SH3 domain; ORF1; SH3 adapter protein SPIN90; SH3 protein interacting with Nck, 90 kDa; SPIN90; VIP54; WASLBP; WASP-interacting SH3-domain protein; WISH; wiskott-Aldrich syndrome protein-interacting protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.