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Primary Antibodies

Rabbit anti-SRC3 Antibody Affinity Purified

Name: Rabbit anti-SRC3 Antibody
Brand: Bethyl Laboratories
SKU: A300-347A
Price: 131 USD

Bethyl Laboratories Catalog #

Citations (8)

Target:

SRC3

Reactivity:

Human

Applications:

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 900 and 950

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00

Qty:

Product Details

Specifications

Verified Reactivity

Human

Antigen Species

Human

Concentration

1000 µg/ml

Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State

Liquid

Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures

Antibody was affinity purified using an epitope specific to SRC3 immobilized on solid support.

The epitope recognized by A300-347A maps to a region between residue 900 and 950 of human Steroid Receptor Coactivator 3 using the numbering given in entry NP_858045.1 (GeneID 8202).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SRC3 immobilized on solid support.

The epitope recognized by A300-347A-T maps to a region between residue 900 and 950 of human Steroid Receptor Coactivator 3 using the numbering given in entry NP_858045.1 (GeneID 8202).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

Steroid receptor coactivator 3 (SRC3) belongs to the SRC family of proteins that interacts with nuclear receptors to facilitate transcription. The 3 homologous members of this family are SRC-1 (NCoA-1), SRC-2 (GRIP1, TIF2, or NcoA-2), and SRC-3 (p/CIP, RAC3, ACTR, AIB1, or TRAM-1). SRC3 is an oncogene that is reported to be amplified and/or overexpressed in breast and gastric cancer and stimulates AKT signaling and cell growth and tumorigenesis.

Gene ID: 8202

Gene Symbol: NCOA3

Gene Name: nuclear receptor coactivator 3

Uniprot ID: Q9Y6Q9

Protein Name: Nuclear receptor coactivator 3

Alternate Names

ACTR; AIB1; AIB-1; amplified in breast cancer 1 protein; bHLHe42; CAGH16; CBP-interacting protein; class E basic helix-loop-helix protein 42; CTG26; KAT13B; NCoA-3; nuclear receptor coactivator 3; pCIP; RAC3; RAC-3; receptor-associated coactivator 3; SRC3; SRC-3; steroid receptor coactivator protein 3; thyroid hormone receptor activator molecule 1; TNRC14; TNRC16; TRAM-1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.