Rabbit anti-SYNE2/Nesprin 2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-392A-T maps to a region between residue 1075 to 1125 of human Nesprin-2 using the numbering given in entry Q8WXH0.3 (GeneID 23224).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SYNE2/Nesprin 2 immobilized on solid support.
The epitope recognized by A305-392A maps to a region between residue 1075 to 1125 of human Nesprin-2 using the numbering given in entry Q8WXH0.3 (GeneID 23224).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
SYNE2/Nesprin 2 is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus [taken from NCBI Entrez Gene (Gene ID: 23224)].
Alternate Names
EDMD5; KASH domain-containing protein 2; KASH2; Nesp2; nesprin 2; Nesprin-2; NUA; NUANCE; nuclear envelope spectrin repeat protein 2; nucleus and actin connecting element protein; polytrophin; Protein NUANCE; spectrin repeat containing, nuclear envelope 2; synaptic nuclear envelope protein 2; synaptic nuclei expressed gene 2; SYNE-2; TROPH
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.