Rabbit anti-SYNM Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-468A maps to a region between residue 1400 to 1450 of human Synemin using the numbering given in entry O15061.2 (GeneID 23336).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to SYNM immobilized on solid support.
The epitope recognized by A305-468A-T maps to a region between residue 1400 to 1450 of human Synemin using the numbering given in entry O15061.2 (GeneID 23336).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Synemin is an intermediate filament (IF) family member. IF proteins are cytoskeletal proteins that confer resistance to mechanical stress and are encoded by a dispersed multigene family. This protein has been found to form a linkage between desmin, which is a subunit of the IF network, and the extracellular matrix, and provides an important structural support in muscle [taken from NCBI Entrez Gene (Gene ID: 23336)].
Alternate Names
desmuslin; DMN; SYN; synemin; synemin alpha; synemin beta; synemin, intermediate filament protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.