Rabbit anti-Synoviolin Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-946A maps to a region between residue 567 and 617 of human Synovial Apoptosis Inhibitor 1 using the numbering given in entry NP_757385.1 (GeneID 84447).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Synoviolin immobilized on solid support.
The epitope recognized by A302-946A-T maps to a region between residue 567 and 617 of human Synovial Apoptosis Inhibitor 1 using the numbering given in entry NP_757385.1 (GeneID 84447).
Additional Product Information
The E3 ubiquitin-protein ligase synoviolin is also known as human HRD1. The original HRD1 (HMG-CoA reductase degradation) gene was identified as a mutant gene in yeast deficient for the degradation of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR). Homology between the human and yeast gene is limited to the N-terminal domain of the proteins, and human HRD1/Synoviolin does not appear to be involved in the degradation of mammalian HMGR. Synoviolin is involved in endoplasmic reticulum (ER)-associated degradation. It removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins.
Alternate Names
DER3; E3 ubiquitin-protein ligase synoviolin; HMG-coA reductase degradation 1 homolog; HRD1; RING-type E3 ubiquitin transferase synoviolin; Synovial apoptosis inhibitor 1; synovial apoptosis inhibitor 1, synoviolin
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.