Rabbit anti-Syntaxin 7 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-512A maps to a region between residue 100 to 150 of human Syntaxin 7 using the numbering given in entry NP_003560.2 (GeneID 8417).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Syntaxin 7 immobilized on solid support.
The epitope recognized by A304-512A-T maps to a region between residue 100 to 150 of human Syntaxin 7 using the numbering given in entry NP_003560.2 (GeneID 8417).
Additional Product Information
Syntaxin 7 may be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Syntaxin 7 mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O15400].
Alternate Names
syntaxin-7
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.