Rabbit anti-T-bet/TBX21 Recombinant Monoclonal Antibody [BLR110H]
Product Details
Specifications
Immunogen was a peptide representing a region between residue 485 and the C-terminus (residue 535) of human T-box transcription factor TBX21 using the numbering given in entry NP_037483.1 (Gene ID 30009).
Additional Product Information
T-bet/TBX21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. TBX21 is a lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and activating transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. [taken from NCBI Entrez Gene (Gene ID: 30009)].
Alternate Names
TBET; T-bet; TBLYM; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; T-PET; transcription factor TBLYM
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.