Rabbit anti-TAF5 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
The TFIID complex is important for coordinating the pre-initiation complex and transcription by RNA Pol II. The complex contains the TFIID protein, TATA binding protein (TBP), and multiple TBP-associated factors (TAFs). TAF5 is an integral subunit of the transcriptionally competent TFIID complex.
Alternate Names
TAF(II)100; TAF2D; TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa; TAFII100; TAFII-100; TATA box binding protein (TBP)-associated factor 2D; TATA box binding protein (TBP)-associated factor, RNA polymerase II, D, 100kD; transcription initiation factor TFIID 100 kD subunit; transcription initiation factor TFIID 100 kDa subunit; transcription initiation factor TFIID subunit 5
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.