Rabbit anti-TAFII68 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
The TFIID complex is important for coordinating the pre-initiation complex and transcription by RNA Pol II. The complex contains the TFIID protein, TATA binding protein (TBP) and multiple TBP-associated factors (TAFs). TAFII68 was identified as a subunit of the TFIID complex, but has also been found to be associated with the Pol II multiprotein complex. TAFII68 is part of the TET family of proteins that can bind RNA and single-stranded DNA. This family also includes the human sarcoma-associated proteins EWS and TLS. The association of this family of proteins with Pol II suggests that they may bridge the processes of transcription initiation and elongation. The N-terminus domain of TAFII68 in gene fusions has been shown to have oncogenic properties, similar to the chromosomal translocation associated with the EWS gene.
Alternate Names
68 kDa TATA-binding protein-associated factor; Npl3; RBP56; RBP56/CSMF fusion; RNA-binding protein 56; TAF(II)68; TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa; TAF2N; TAFII68; TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA-binding protein 56); TATA box-binding protein-associated factor 2N (RNA-binding protein 56); TATA-binding protein-associated factor 2N; TBP-associated factor 15
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.