Rabbit anti-TAK1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-916A maps to a region between residue 450 and 500 of human transforming growth factor-beta-activated kinase 1 using the numbering given in entry NP_663304.1 (GeneID 6885).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TAK1 immobilized on solid support.
The epitope recognized by A301-916A-T maps to a region between residue 450 and 500 of human transforming growth factor-beta-activated kinase 1 using the numbering given in entry NP_663304.1 (GeneID 6885).
Additional Product Information
TAK1 (TGF-beta activated kinase 1) is also called mitogen-activated protein kinase kinase kinase 7 (MAP3K7). TAK1 is a serine/threonine protein kinase that mediates signals transduced by proinflammatory cytokines such as TGF-beta, tumor necrosis factor (TNF), interleukin-1 (IL-1) and wnt family ligands. TAK1 transduces signals by stimulating a MAPK pathway that ultimately stimulates JNK (c-terminal jun kinase) and p38 MAPKs. TAK1 is also an activator of the Ikappa B kinase that leads to the activation of NFkappa-B.
Alternate Names
CSCF; FMD2; MEKK7; mitogen-activated protein kinase kinase kinase 7; TAK1; TGF1a; TGF-beta activated kinase 1; TGF-beta-activated kinase 1; transforming growth factor-beta-activated kinase 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.