Rabbit anti-TCF12 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-755A maps to a region between residue 50 and 100 of human Transcription Factor 12 using the numbering given in entry NP_996919.1 (GeneID 6938).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TCF12 immobilized on solid support.
The epitope recognized by A300-755A-T maps to a region between residue 50 and 100 of human Transcription Factor 12 using the numbering given in entry NP_996919.1 (GeneID 6938).
Additional Product Information
TCF12 (transcription factor 12), also known as HeLa E-box binding protein (HEB), is basic helix-loop-helix (bHLH) transcription factor that recognizes consensus E-box binding sites and is found to be important for T-cell development and cell differentiation. TCF12 can interact with the immunoglobulin enhancer binding proteins E12 and ITF2 as hetero-oligomers to modulate their DNA binding activity.
Alternate Names
bHLHb20; class B basic helix-loop-helix protein 20; CRS3; DNA-binding protein HTF4; E-box-binding protein; HEB; helix-loop-helix transcription factor 4; HsT17266; HTF4; p64; TCF-12; transcription factor 12; transcription factor HTF-4
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.