Rabbit anti-TDP43 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
TDP43
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 175 and 225
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to TDP43 immobilized on solid support.

The epitope recognized by A303-223A maps to a region between residue 150 and 200 of human TAR DNA-Binding Protein 43 using the numbering given in entry NP_031401.1 (GeneID 23435).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to TDP43 immobilized on solid support.

The epitope recognized by A303-223A-T maps to a region between residue 150 and 200 of human TAR DNA-Binding Protein 43 using the numbering given in entry NP_031401.1 (GeneID 23435).
Country of Origin
USA

Additional Product Information

Tar DNA-binding protein 43 (TDP43) is a DNA and RNA-binding protein which regulates transcription and splicing. It is involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. TDP43 may also be involved in microRNA biogenesis, apoptosis and cell division. TDP43 can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Also, TDP43 stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q13148].

23435
TARDBP
TAR DNA binding protein
Q13148
TAR DNA-binding protein 43

Alternate Names

ALS10; TAR DNA-binding protein 43; TAR DNA-binding protein-43; TDP-43

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.