Rabbit anti-THEMIS Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Thymocyte-expressed molecule involved in selection (THEMIS) plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells [taken from NCBI Entrez Gene (Gene ID: 387357)].
Alternate Names
C6orf190; C6orf207; GASP; GRB2-associated protein; protein THEMIS; signaling phosphoprotein specific for T cells; SPOT; thymocyte selection pathway associated; thymocyte-expressed molecule involved in selection; TSEPA
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.