Rabbit anti-THOC2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-630A maps to a region between residue 1543 to 1593 of human THO Complex 2 using the numbering given in entry NP_001075019.1 (GeneID 57187).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to THOC2 immobilized on solid support.
The epitope recognized by A303-630A-T maps to a region between residue 1543 to 1593 of human THO Complex 2 using the numbering given in entry NP_001075019.1 (GeneID 57187).
Additional Product Information
THO complex subunit 2 (THOC2) is a component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. THOC2 binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q8NI27].
Alternate Names
CXorf3; dJ506G2.1; hTREX120; mental retardation, X-linked 12; mental retardation, X-linked 35; MRX12; MRX35; THO complex subunit 2; THO2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.