Rabbit anti-ThPOK Recombinant Monoclonal Antibody [BLR191J]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
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Immunogen was a peptide representing a region between residue 250 and 300 of human ThPOK (aka Zinc finger and BTB domain-containing protein 7B) using the numbering given in entry NP_001243384.1 (Gene ID 51043).
Additional Product Information
ZBTB7B is a transcription factor that acts as a key regulator of lineage commitment of immature T-cell precursors. ZBTB7B is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. ZBTB7B is also a transcriptional repressor of the collagen COL1A1 and COL1A2 genes and may also function as a repressor of fibronectin and possibly other extracellular matrix genes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ O15156].
Alternate Names
CKROX; c-KROX; hcKROX; krueppel-related zinc finger protein cKrox; T-helper-inducing POZ/Krueppel-like factor; THPOK; ZBTB15; ZFP67; ZFP-67; zinc finger and BTB domain containing 15; Zinc finger and BTB domain-containing protein 15; zinc finger and BTB domain-containing protein 7B; zinc finger protein 67 homolog; zinc finger protein 857B; zinc finger protein Th-POK; ZNF857B
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.