Rabbit anti-TOPORS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-179A maps to a region between residue 995 and 1045 of human topoisomerase I binding, arginine/serine-rich using the numbering given in entry topoisomerase I binding, arginine/serine-rich NP_005793.2 (GeneID 10210).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TOPORS immobilized on solid support.
The epitope recognized by A302-179A-T maps to a region between residue 995 and 1045 of human topoisomerase I binding, arginine/serine-rich using the numbering given in entry topoisomerase I binding, arginine/serine-rich NP_005793.2 (GeneID 10210).
Additional Product Information
TOPORS (topoisomerase I-binding RS protein) was identified in a a yeast two-hybrid screen for proteins that bind the N-terminus of topoisomerase I. TOPORS contains a RING-type zinc-finger domain and an RS-rich region. TOPORS has been found to possess both ubiquitin and SUMO-1 E3 ligase activity. It can ubiquitinate and sumoylate p53 and may possibly function as a tumor suppressor. Defects in TOPORS are the cause of retinitis pigmentosa type 31.
Alternate Names
E3 ubiquitin-protein ligase Topors; LUN; p53-binding protein 3; P53BP3; RING-type E3 ubiquitin transferase Topors; RP31; SUMO1-protein E3 ligase Topors; topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase; Topoisomerase I-binding arginine/serine-rich protein; topoisomerase I-binding RING finger protein; TP53BPL; tumor suppressor p53-binding protein 3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.