Rabbit anti-TRAF4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-840A maps to a region between residue 225 and 275 of human TNF Receptor-Associated Factor 4 using the numbering given in entry NP_004286.2 (GeneID 9618).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TRAF4 immobilized on solid support.
The epitope recognized by A302-840A-T maps to a region between residue 225 and 275 of human TNF Receptor-Associated Factor 4 using the numbering given in entry NP_004286.2 (GeneID 9618).
Additional Product Information
TNF receptor-associated factor 4 (TRAF4) is an adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. TRAF4 seems to mediate activation of NF-kappa-B and JNK and to be involved in apoptosis. TRAF4 may play a role in the development of respiratory tract. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BUZ4].
Alternate Names
CART1; cysteine-rich domain associated with ring and TRAF domain; cysteine-rich domain associated with RING and Traf domains protein 1; malignant 62; metastatic lymph node gene 62 protein; MLN 62; MLN62; RING finger protein 83; RNF83; TNF receptor-associated factor 4; TRAF4 variant 6; tumor necrosis receptor-associated factor 4A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.