Rabbit anti-TRAF6 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-803A maps to a region between residue 472 and 522 of human TNF Receptor-Associated Factor 6 using the numbering given in entry NP_004611.1 (GeneID 7189).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TRAF6 immobilized on solid support.
The epitope recognized by A303-803A-T maps to a region between residue 472 and 522 of human TNF Receptor-Associated Factor 6 using the numbering given in entry NP_004611.1 (GeneID 7189).
Additional Product Information
TNF receptor-associated factor 6 (TRAF6) is an E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. TRAF6 also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. TRAF6 leads to the activation of NF-kappa-B and JUN. TRAF6 may be essential for the formation of functional osteoclasts, and it regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. TRAF6 also seems to play a role in dendritic cells (DCs) maturation and/or activation. TRAF6 represses c-Myb-mediated transactivation, in B-lymphocytes. TRAF6 is an adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y4K3].
Alternate Names
E3 ubiquitin-protein ligase TRAF6; interleukin-1 signal transducer; MGC:3310; RING finger protein 85; RING-type E3 ubiquitin transferase TRAF6; RNF85; TNF receptor-associated factor 6; TNF receptor-associated factor 6, E3 ubiquitin protein ligase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).