Rabbit anti-TRIM37 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-174A-T maps to a region between residue 914 and 964 of human tripartite motif-containing 37 using the numbering given in entry NP_056109.1 (GeneID 4591). Antibody was affinity purified using an epitope specific to TRIM37 immobilized on solid support.
The epitope recognized by A301-174A maps to a region between residue 914 and 964 of human tripartite motif-containing 37 using the numbering given in entry NP_056109.1 (GeneID 4591).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
TRIM37 is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM37 is a peroxisomal protein whose function has not been determined. Mutations in TRIM37 result in mulibrey (MUscle, LIver, BRain, and EYes) nanism (‘dwarfism’), a rare autosomal recessive disorder characterized by defects in tissues of mesodermal origin.
Alternate Names
E3 ubiquitin-protein ligase TRIM37; MUL; mulibrey nanism protein; POB1; RING-B-box-coiled-coil protein; RING-type E3 ubiquitin transferase TRIM37; TEF3; Tripartite motif-containing protein 37
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.