Rabbit anti-TRIP12 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-814A maps to a region between residue 75 and 125 of human thyroid hormone receptor interactor 12 using the numbering given in entry NP_004229.1 (GeneID 9320).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TRIP12 immobilized on solid support.
The epitope recognized by A301-814A-T maps to a region between residue 75 and 125 of human thyroid hormone receptor interactor 12 using the numbering given in entry NP_004229.1 (GeneID 9320).
Additional Product Information
TRIP12 (thyroid hormone receptor interactor 12) was originally identified as a factor that interacts with the thyroid hormone receptor. It was later found to also interact with AAP-BP1, a component of the NEDD8 –activating enzyme, and function as an E3 ubiquitin ligase for AAP-BP1.
Alternate Names
E3 ubiquitin-protein ligase for Arf; E3 ubiquitin-protein ligase TRIP12; HECT-type E3 ubiquitin transferase TRIP12; MRD49; probable E3 ubiquitin-protein ligase TRIP12; thyroid receptor interacting protein 12; Thyroid receptor-interacting protein 12; TR-interacting protein 12; TRIP-12; ULF
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.