Rabbit anti-TRIP4/ASC1-1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-203A maps to a region between residues 75 and 125 of human thyroid hormone receptor interactor 4, (activating signal cointegrator 1) using the number given in entry NP_057297.2 (GeneID 9325).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TRIP4/ASC-1 immobilized on solid support.
The epitope recognized by A300-203A-T maps to a region between residues 75 and 125 of human thyroid hormone receptor interactor 4, (activating signal cointegrator 1) using the number given in entry NP_057297.2 (GeneID 9325).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Thyroid receptor interacting protein 4 (TRIP4) is also known as activating signal cointegrator 1 (ASC-1). TRIP4/ASC-1 was identified as a protein that interacts with the thyroid receptor. It has been shown to act as a transcriptional co-activator of the thyroid receptor (TR), 9-cis retinoic acid (RXR), androgen receptor (AR) and estrogen.
Alternate Names
activating signal cointegrator 1; ASC1; ASC-1; HsT17391; MDCDC; SMABF1; thyroid receptor-interacting protein 4; TR-interacting protein 4; TRIP-4; ZC2HC5; zinc finger, C2HC5-type
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.