Rabbit anti-TRMT61A Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-858A maps to a region between residue 239 and 289 of human tRNA using the numbering given in entry Q96FX7.1 (GeneID 115708).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to TRMT61A immobilized on solid support.
The epitope recognized by A305-858A-T maps to a region between residue 239 and 289 of human tRNA using the numbering given in entry Q96FX7.1 (GeneID 115708).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
TRMT61A is the catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Catalytic subunit of mRNA N(1)-methyltransferase complex, which mediates methylation of adenosine residues at the N(1) position of a small subset of mRNAs: N(1) methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96FX7].
Alternate Names
C14orf172; GCD14; Gcd14p; hTRM61; mRNA methyladenosine-N(1)-methyltransferase catalytic subunit TRMT61A; TRM61; tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A; tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61; tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT61A; tRNA methyltransferase 61 homolog A; tRNA(m1A58)-methyltransferase subunit TRM61; tRNA(m1A58)-methyltransferase subunit TRMT61A; tRNA(m1A58)MTase subunit TRM61; tRNA(m1A58)MTase subunit TRMT61A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).