Rabbit anti-U2AF35 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-079A maps to a region between residue 140 and 190 of human U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit using the numbering given in entry NP_006749.1 (GeneID 7307).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
U2AF35 heterodimerizes with U2AF65 to form the U2 snRNP auxiliary factor which is part of the splicesome. U2AF35 is required for splicing of AG-dependent pre-mRNAs and functions in recognizing the conserved 3’ splice site dinucleotide AG. U2AF35 contains an atypical RNA recognition motif (RRM), two C3H1-type zinc fingers, and a C-terminal arginine/serine (RS)-rich domain interrupted by glycines.
Alternate Names
FP793; RN; RNU2AF1; splicing factor U2AF 35 kDa subunit; splicing factor U2AF 35kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit; U2 small nuclear RNA auxiliary factor 1; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein; U2AF35; U2AFBP
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.