Rabbit anti-UAP1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-642A maps to a region between residue 1 to 50 of human UDP-N-acetylhexosamine pyrophosphorylase using the numbering given in entry Q16222.3 (GeneID 6675).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to UAP1 immobilized on solid support.
The epitope recognized by A305-642A-T maps to a region between residue 1 to 50 of human UDP-N-acetylhexosamine pyrophosphorylase using the numbering given in entry Q16222.3 (GeneID 6675).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q16222].
Alternate Names
AGX; AGX1; AGX-1; AGX2; AGX-2; antigen X; SPAG2; sperm associated antigen 2; Sperm-associated antigen 2; testis tissue sperm-binding protein Li 37a; UDP-N-acetylgalactosamine pyrophosphorylase; UDP-N-acetylglucosamine diphosphorylase 1; UDP-N-acetylglucosamine pyrophosphorylase; UDP-N-acetylhexosamine pyrophosphorylase; UDP-N-acetylhexosamine pyrophosphorylase 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.