Rabbit anti-UBE2O Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-873A maps to a region between residue 725 and 775 of human ubiquitin-conjugating enzyme E2O using the numbering given in entry NP_071349.2 (GeneID 63893).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to UBE2O immobilized on solid support.
The epitope recognized by A301-873A-T maps to a region between residue 725 and 775 of human ubiquitin-conjugating enzyme E2O using the numbering given in entry NP_071349.2 (GeneID 63893).
Additional Product Information
UBE2O (ubiquitin-conjugating enzyme E2O) is an E2 ubiquitin conjugating enzyme that is part of the three-step pathway responsible for the covalent attachment of ubiquitin to proteins for their targeted degradation.
Alternate Names
(E3-independent) E2 ubiquitin-conjugating enzyme; E2/E3 hybrid ubiquitin-protein ligase UBE2O; E2-230K; ubiquitin carrier protein O; ubiquitin conjugating enzyme E2O; Ubiquitin-conjugating enzyme E2 O; ubiquitin-conjugating enzyme E2 of 230 kDa; ubiquitin-conjugating enzyme E2-230K; ubiquitin-protein ligase O
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.