Rabbit anti-UBE3C Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Ubiquitin protein ligase E3C (UBE3C) is an E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15386].
Alternate Names
HECTH2; HECT-type ubiquitin transferase E3C; ubiquitin-protein isopeptide ligase (E3); ubiquitin-protein ligase E3C
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.