Rabbit anti-UBE4B Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-124A-T maps to a region between residue 1252 and 1302 of human ubiquitination factor E4B using the numbering given in entry AAD02233.1 (GeneID 10277).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to UBE4B immobilized on solid support.
The epitope recognized by A301-124A maps to a region between residue 1252 and 1302 of human ubiquitination factor E4B using the numbering given in entry AAD02233.1 (GeneID 10277).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Ubiquitin conjugation factor E4 B (UBE4B) is an E4 enzyme of the multi-step ubiquitination pathway. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes and the ligation of ubiquitin to the target protein by E3 enzymes. The E4 enzyme is an additional conjugation factor which is involved in polyubiquitin chain assembly. UBE4B has been identified as a candidate neuroblastoma tumor suppressor gene.
Alternate Names
E4; HDNB1; homologous to yeast UFD2; Homozygously deleted in neuroblastoma 1; homozygously deleted in neuroblastoma-1; RING-type E3 ubiquitin transferase E4 B; ubiquitin conjugation factor E4 B; Ubiquitin fusion degradation protein 2; ubiquitination factor E4B (UFD2 homolog, yeast); ubiquitin-fusion degradation protein 2; UBOX3; UFD2; UFD2A; UFD2A-III/UBE4B-III splice isoform
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.