Rabbit anti-UCH37 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Ubiquitin C-terminal hydrolase UCH37 is a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. UCH37 is a deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. It is a putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y5K5].
Alternate Names
CGI-70; INO80 complex subunit R; INO80R; ubiquitin carboxyl-terminal esterase L5; ubiquitin carboxyl-terminal hydrolase isozyme L5; ubiquitin carboxyl-terminal hydrolase L5; ubiquitin C-terminal hydrolase UCH37; ubiquitin thioesterase L5; UCH37; UCH-L5
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.