Rabbit anti-USF2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-541A maps to a region between residue 150 and 200 of human Upstream Transcription Factor 2 using the numbering given in entry NP_003358.1 (GeneID 7392).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to USF2 immobilized on solid support.
The epitope recognized by A303-541A-T maps to a region between residue 150 and 200 of human Upstream Transcription Factor 2 using the numbering given in entry NP_003358.1 (GeneID 7392).
Additional Product Information
Upstream stimulatory factor 2 (USF2) is a transcription factor that binds to a symmetrical DNA sequence (E-box) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15853].
Alternate Names
bHLHb12; c-fos interacting protein; class B basic helix-loop-helix protein 12; FIP; FOS-interacting protein; major late transcription factor 2; upstream stimulatory factor 2; Upstream transcription factor 2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.