Rabbit anti-USP20/VDU2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-189A maps to a region between residue 863 and 913 of human ubiquitin specific peptidase 20 (pVHL-interacting deubiquitinating enzyme 2) using the numbering given in entry NP_006667.2 (GeneID 10868).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to USP20/VDU2 immobilized on solid support.
The epitope recognized by A301-189A-T maps to a region between residue 863 and 913 of human ubiquitin specific peptidase 20 (pVHL-interacting deubiquitinating enzyme 2) using the numbering given in entry NP_006667.2 (GeneID 10868).
Additional Product Information
The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific peptidase 20 (USP20) is a UBP and is also called von-Hippel-Lindau protein (pVHL) interacting deubiquitinating enzyme 2 (VDU2). pVHL is an E3 ubiquitin-ligase that ubiquitinates hypoxia-inducible factor (HIF)-1alpha. USP20/VDU2 functions to specifically deubiquitinate HIF-1alpha to increase expression of HIF-1alpha regulated genes.
Alternate Names
deubiquitinating enzyme 20; hVDU2; LSFR3A; pVHL-interacting deubiquitinating enzyme 2; ubiquitin carboxyl-terminal hydrolase 20; ubiquitin thioesterase 20; ubiquitin thiolesterase 20; ubiquitin-specific-processing protease 20; VDU2; VHL-interacting deubiquitinating enzyme 2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.