Rabbit anti-V5 Tag Recombinant Monoclonal Antibody [BLRE03H]
Product Details
Specifications
Immunogen was a peptide representing the V5 Epitope tag (GKPIPNPLLGLDST).
Additional Product Information
The V5 epitope tag is an epitope tag with a 14-residue sequence of GKPIPNPLLGLDST. Derived from the RNA polymerase alpha subunit of simian parainfluenza virus 5, V5 tags can help with detection of proteins in western blot, IP, or microscopic techniques. also especially small and are less likely to interfere with protein function.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.