Rabbit anti-VCIP135 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-933A maps to a region between residue 1100 and 1150 of human Valosin-Containing Protein (p97)/p47 Complex-Interacting Protein p135 using the numbering given in entry NP_079330.2 (GeneID 80124).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to VCIP135 immobilized on solid support.
The epitope recognized by A302-933A-T maps to a region between residue 1100 and 1150 of human Valosin-Containing Protein (p97)/p47 Complex-Interacting Protein p135 using the numbering given in entry NP_079330.2 (GeneID 80124).
Additional Product Information
VCIP135 (valosin-containing protein p97/p47 complex-interacting protein p135) acts as a deubiquitinating enzyme. VCIP135 is necessary for VCP-mediated reassembly of Golgi stacks after mitosis and may play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). VCIP135 also mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q96JH7].
Alternate Names
deubiquitinating protein VCIP135; DUBA3; valosin-containing protein p97/p47 complex-interacting protein 1; valosin-containing protein p97/p47 complex-interacting protein p135; VCIP135; VCP/p47 complex-interacting 135-kDa protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.