Rabbit anti-VCP Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-588A maps to a region between residues 1 and 50 of human Valosin-Containing Protein using the numbering given in entry NP_009057.1 (GeneID 7415).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to VCP immobilized on solid support.
The epitope recognized by A300-588A-T maps to a region between residues 1 and 50 of human Valosin-Containing Protein using the numbering given in entry NP_009057.1 (GeneID 7415).
Additional Product Information
Valosin-containing protein (VCP) is a multifunctional protein that is proposed to be involved in vesicle transport and fusion for protein trafficking, 26S proteasome function in ubiquitin-based degradation pathways, and the assembly of peroxisomes. Mutations in the human VCP gene have been associated with diseases such as inclusion body myopathy, Paget disease of the bone, and frontotemporal dementia (IBMPFD). VCP can inhibit apoptosis and may play a role in tumorigenesis.
Alternate Names
15S Mg(2+)-ATPase p97 subunit; CDC48; p97; TER ATPase; TERA; transitional endoplasmic reticulum ATPase; Valosin-containing protein; VCP
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.