Rabbit anti-VISTA Recombinant Monoclonal Antibody [BLR035F]
Product Details
Specifications
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Immunogen was a peptide representing a region between residue 250 and the C-terminus (residue 311) of human V-type immunoglobulin domain-containing suppressor of T-cell activation precursor using the numbering given in entry NP_071436.1 (Gene ID 64115). Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 250 and the C-terminus (residue 311) of human V-type immunoglobulin domain-containing suppressor of T-cell activation precursor using the numbering given in entry NP_071436.1 (Gene ID 64115).
Additional Product Information
VISTA is an immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993). It may promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). VISTA may stimulate MMP14-mediated MMP2 activation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H7M9].
Alternate Names
B7H5; B7-H5; C10orf54; DD1alpha; Death Domain1alpha; GI24; PD-1H; PDCD1 homolog; platelet receptor GI24; PP2135; SISP1; sisp-1; stress-induced secreted protein-1; V-domain Ig suppressor of T cell activation; VISTA; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; V-type immunoglobulin domain-containing suppressor of T-cell activation
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.