Rabbit anti-vPARP Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-954A maps to a region between residue 1674 to 1724 of human vault poly(ADP-ribose) polymerase using the numbering given in entry NP_006428.2 (GeneID 143).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to vPARP immobilized on solid support.
The epitope recognized by A301-954A-T maps to a region between residue 1674 to 1724 of human vault poly(ADP-ribose) polymerase using the numbering given in entry NP_006428.2 (GeneID 143).
Additional Product Information
PARP4 [poly(ADP-ribose) polymerase family, member 4] catalyzes the formation of ADP-ribose polymers covalently attached to proteins using NAD+ as a substrate. There are 6 mammalian PARP enzymes: PARP1, PARP2, PARP3, PARP4/vPARP, PARP5/Tankyrase-1, and PARP6/Tankyrase-2. PARP4 is also known as vault PARP (vPARP) due to its association with the vault particle, a 13MDa cytoplasmic ribonucleoprotein complex whose barrel-shaped structure resembles arched cathedral ceilings. vPARP has also been identified separate from the vault particle. It has been localized to the nuclear pore, mitotic spindle, and cytoplasm. It also has been shown to associate with telomerase activity. Studies of vPARP knockout mice show an increased susceptibility to carcinogen-induced tumorigenesis.
Alternate Names
193 kDa vault protein; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 1; ADP-ribosyltransferase diphtheria toxin-like 4; ADPRTL1; ARTD4; H5 proline-rich; I-alpha-I-related; p193; PARP-4; PARPL; PARP-related; PARP-related/IalphaI-related H5/proline-rich; PH5P; poly ADP-ribose polymerase 4; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase-like 1; vault poly(ADP-ribose) polymerase; vault protein, 193-kDa; VAULT3; von Willebrand factor A domain containing 5C; VPARP; VWA5C
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.