Rabbit anti-WARS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-275A maps to a region between residue 421 to 471 of human Tryptophanyl-tRNA Synthetase using the numbering given in entry NP_004175.2 (GeneID 7453).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to WARS immobilized on solid support.
The epitope recognized by A304-275A-T maps to a region between residue 421 to 471 of human Tryptophanyl-tRNA Synthetase using the numbering given in entry NP_004175.2 (GeneID 7453).
Additional Product Information
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family [taken from NCBI Entrez Gene (Gene ID: 7453)].
Alternate Names
epididymis secretory sperm binding protein; GAMMA-2; HMN9; hWRS; IFI53; IFP53; interferon-induced protein 53; trpRS; tryptophan tRNA ligase 1, cytoplasmic; tryptophan--tRNA ligase, cytoplasmic; Tryptophanyl-tRNA synthetase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.