Rabbit anti-XPD Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-658A-T maps to a region between residue 710 and 760 of human Xeroderma Pigmentosum Complementary Group D, using the numbering given in entry NP_000391.1 (GeneID 2068). Antibody was affinity purified using an epitope specific to XPD immobilized on solid support.
The epitope recognized by A303-658A maps to a region between residue 710 and 760 of human Xeroderma Pigmentosum Complementary Group D, using the numbering given in entry NP_000391.1 (GeneID 2068).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
XPD (Xeroderma pigmentosum group D-complementing protein) is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. XPD has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in the XPD gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome [taken from NCBI Entrez Gene (Gene ID: 2068)].
Alternate Names
basic transcription factor 2 80 kDa subunit; BTF2 p80; COFS2; CXPD; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; EM9; excision repair cross-complementation group 2; TFIIH; TFIIH basal transcription factor complex 80 kDa subunit; TFIIH p80; TFIIH subunit XPD; TTD; TTD1; xeroderma pigmentosum complementary group D; XPD
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.