Rabbit anti-YTHDC1/YT521 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-096A maps to a region between residue 50 to 100 of human YTH domain-containing protein 1 using the numbering given in entry Q96MU7.3 (GeneID 91746).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to YTHDC1/YT521 immobilized on solid support.
The epitope recognized by A305-096A-T maps to a region between residue 50 to 100 of human YTH domain-containing protein 1 using the numbering given in entry Q96MU7.3 (GeneID 91746).
Additional Product Information
YTH domain-containing protein 1, encoded by the gene YTHDC1, specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs and regulates alternative splice site selection [taken from UniProtKB (Entry: Q96MU7)].
Alternate Names
putative splicing factor YT521; splicing factor YT521; splicing factor YT521-B; YT521; YT521-B; YTH domain-containing protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.