Rabbit anti-YY1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-779A-T maps to a region between residue 50 and 100 of human Yin and Yang 1 Protein using the numbering given in entry NP_003394.1 (GeneID 7528). Antibody was affinity purified using an epitope specific to YY1 immobilized on solid support.
The epitope recognized by A302-779A maps to a region between residue 50 and 100 of human Yin and Yang 1 Protein using the numbering given in entry NP_003394.1 (GeneID 7528).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
YY1 (ying and yang 1) is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
Alternate Names
DELTA; delta transcription factor; GADEVS; INO80 complex subunit S; INO80S; NF-E1; transcriptional repressor protein YY1; UCRBP; Yin and yang 1; Yin and Yang 1 protein; YIN-YANG-1; YY-1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.