Rabbit anti-ZONAB Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-070A maps to a region between residue 250 and 300 of human ZO-1-associated nucleic acid-binding protein using the numbering given in entry NP_003642.3 (GeneID 8531).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ZONAB immobilized on solid support.
The epitope recognized by A303-070A-T maps to a region between residue 250 and 300 of human ZO-1-associated nucleic acid-binding protein using the numbering given in entry NP_003642.3 (GeneID 8531).
Additional Product Information
ZONAB is also known as CSDA (cold shock domain-containing protein A). ZONAB binds to the GM-CSF promoter and appears to act as a repressor. ZONAB also binds to full length mRNA and to short RNA sequences containing the consensus site 5'-UCCAUCA-3’ and may have a role in translation repression.
Alternate Names
cold shock domain-containing protein A; cold-shock domain containing A1; cold-shock domain protein A; CSDA; CSDA1; DBPA; DNA-binding protein A; single-strand DNA-binding protein NF-GMB; Y-box-binding protein 3; ZO-1-associated nucleic acid-binding protein; ZONAB
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.