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Sheep anti-Bovine Albumin Cross-Adsorbed Antibody Affinity Purified
Bethyl Laboratories
Catalog #
Target:
Bovine Albumin
Reactivity:
Bovine
Applications:
ELISA,
ICC,
IHC,
WB
Host:
Sheep
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
HRP,
Unconjugated
Purity:
Antigen Affinity Purified
Product Details
Specifications
Verified Reactivity
Bovine
Antigen Species
Bovine
Minimal Reactivity
Human, Mouse, Pig
Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
2 years from date of receipt,
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.05% Pro-Clean 400
Request Formulation Change
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.05% Pro-Clean 400
Request Formulation Change
Production Procedures
Antiserum was cross adsorbed using human, mouse and pig immunosorbents to remove cross reactive Antibodies. The antibody to bovine albumin was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine albumin. Less than 1% cross reactivity to human, mouse and pig albumin was detected. This antibody may cross react with albumin from other species. Antiserum was cross adsorbed using human, mouse and pig immunosorbents to remove cross reactive Antibodies. The antibody to bovine albumin was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme:antibody protein ratio is 1:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine albumin. Less than 1% cross reactivity to human, mouse and pig albumin was detected. This antibody may cross react with albumin from other species.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine albumin. Less than 1% cross reactivity to human, mouse and pig albumin was detected. This antibody may cross react with albumin from other species. Antiserum was cross adsorbed using human, mouse and pig immunosorbents to remove cross reactive Antibodies. The antibody to bovine albumin was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme:antibody protein ratio is 1:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine albumin. Less than 1% cross reactivity to human, mouse and pig albumin was detected. This antibody may cross react with albumin from other species.
Country of Origin
USA
Additional Product Information
Albumin is a serum protein responsible for pH maintenance and the transport of a variety of compounds, such as fatty acids, amino acids, and hormones. It is also capable of binding to water molecules and modulates oncotic pressure.
Applications
Not all listed applications have been specifically tested by our laboratory.