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Tyramide Signal Amplification (TSA)-Based Immunofluorescent Multiplex (mIF) AssaysProtocolMultiplex immunofluorescent (mIF) assays provide information on the expression levels of biomarkers. This protocol gives you the guidelines to optimize immunofluorescence assays.
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Cell Lysate Preparation - NETN Buffer MethodProtocolThis protocol outlines cell lysate preparation using the NETN method. This includes the recipes for reagents and the procedures for adherent cells and suspension culture.
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Cell Lysate Preparation - Nuclear ExtractProtocolExtracts prepared from the isolated nuclei of cultured cells can be used in functional studies and for the purification of proteins. This protocol adapted from Mirmira Lab, University of Virginia outlines the steps and processes required.
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Cell Lysate Preparation - RIPA Buffer MethodProtocolRIPA (Radioimmunoprecipitation Assay) - Buffer is a reagent used in cell lysis experimentation, to enable rapid, efficient solubilization of proteins. By minimizing non-specific protein binding, specific binding interactions can be easily studied and are commonly used in immunoprecipitation experiments.
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Fixation and Permeabilization of Whole Blood with Red Blood Cell LysisProtocolFind out more about the reagents required alongside the cell fixation and permeabilization protocol when dealing with red blood cell (RBC) lysis of whole blood.