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From Our Learning Center, Discover All Antibody Resources Including Webinars, eBooks, and Protocols.

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From Our Learning Center, Discover All Cell & Gene Therapy Resources Including Webinars, eBooks, and Protocols.

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Western Blot Protocols | Fortis Life Sciences

Western Blot Protocols

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Immunoprecipitation Protocols | Fortis Life Sciences

Immunoprecipitation Protocols

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ELISA Protocols | Fortis Life Sciences

ELISA Protocols

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Choosing the correct Antigen Proteins is important for any Custom Antibody project. Learn more about Fortis Life Sciences today!

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Find all Immunoassay Buffers From Fortis Life Sciences. Made In The USA.

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Find all ELISA Kits & Accessories from Fortis Life Sciences. Featuring High Quality, Validated Antibodies Made In The USA.

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Find all ELISA Accessories from Fortis Life Sciences. This includes a range of wash, blocking and coating buffer solutions as well as microtiter plates.

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Find all PathPlex® IHC Panels from Fortis Life Sciences. Featuring High Quality, Validated Antibodies Made In The USA.

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PathPlex® IHC Panels

Fortis Life Sciences provides high-quality reverse transcription (RT) kits developed for reliable results in RT-PCR and RT-qPCR applications. Our solutions support cDNA synthesis and one-step RT-qPCR, enabling critical research in gene expression, pathogen detection, and diagnostic assay development.

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High-quality PCR master mixes for molecular biology applications. Standard, hot-start, qPCR, & inhibitor-tolerant formulations. Bulk & custom options.

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Find all All Antibodies from Fortis Life Sciences. High Quality, Validated Antibodies Made In The USA.

All Antibodies | Fortis Life Sciences

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Fortis Life Sciences offers a range of standard and custom monoclonal antibodies, available for a wide range of immunoassay applications.

Monoclonal Antibodies (mAbs) - Fortis

Monoclonal Antibodies

Polyclonal Antibodies (pAbs) - Fortis

Polyclonal Antibodies

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Lateral Flow Antibodies, Antigens & Gold Conjugates

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Apoptosis Pathway Antibodies | Fortis Life Sciences

Apoptosis Pathway Antibodies

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Antibodies by Research Area | Fortis Life Sciences

Antibodies by Research Area

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Antibodies & Antigens | Fortis Life Sciences

Antibodies & Antigens

Find all AbNano™ VHH Libraries From Fortis Life Sciences. Featuring High Quality, Validated Antibodies Made In The USA.

AbNano™ VHH Libraries | Fortis Life Sciences

AbNano™ VHH Libraries

Find all Libraries From Fortis Life Sciences. Featuring High Quality, Validated Antibodies Made In The USA.

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Libraries

Products

Advantages of Recombinant Monoclonal Antibodies over Hybridoma-Derived Antibodies

Recombinant monoclonal antibodies have emerged as a superior alternative to hybridoma-derived monoclonal antibodies, offering numerous advantages in antibody production and application. This article explores the benefits of recombinant techniques over traditional hybridoma methods, focusing on considerations such as reproducibility, scalability, storage, and the increasing role of recombinant antibodies in diagnostics and therapeutics. 

Resource Type

Field

Holliday junctions are a four-way DNA junction formed during replication fork regression, homology-dependent repair, and mitosis. Specifically, they are an intermediate of replication and form at the site of double-stranded DNA breaks.

Happy Hollidays!

Holliday junctions were discovered in 1964 by geneticist Robin Holliday to describe how gene-conversion events occur during meiosis in fungi. Holliday junctions are a four-way DNA junction formed during replication fork regression, homology-dependent repair, and mitosis. Specifically, they are an intermediate of replication and form at the site of double-stranded DNA breaks.

Research Area

Comparing and contrasting antiserum and IgG fractions can guide scientists searching for the right reagent to meet immunoassay-specific needs.

IgG Fractions:  Enrich Affinity and Reduce Background

Development and application of an end-to-end staining and analysis pipeline to identify immune cell infiltrates in oral cancer samples using a targeted multiplex immunohistochemistry antibody panel

The tumor microenvironment (TME) plays a critical role in determining how a tumor may respond to therapy, particularly for cancer immunotherapy approaches. Improvements in the number of biomarkers that can be simultaneously screened have led to the ability to probe for numerous cell types expressing multiple markers within the TME. The ability to probe the tumor microenvironment (TME) at the single cell level is important for understanding of interactions between tumors and immune cells, particularly whether immune cells have infiltrated into tumor nests, and with regards to the presence or absence of immune checkpoint markers. The ability to interrogate these questions relies on the ability to assess multiple biomarkers simultaneously for cell phenotyping in their spatial context. Here we demonstrate rapid design and optimization of a panel of antibodies for multiplexed immunohistochemical staining of a series of oral cancer tumor samples.

Applications

Author

Utilizing the AbNano™ VHH Naïve Library for identifying and characterizing VHH binders to key immuno-oncology targets

VHH domains, derived from the variable heavy chain of the heavy chain-only IgG2 and IgG3 domains in camelids, represent a small, single-domain antibody fragment. Discovering novel VHH domains can take place by multiple workflows, including using B-cell sorting and using display libraries. Within display libraries, the library is generally one of three classes of molecule: synthetic molecules, immune-derived molecules, or naïve germline molecules.<div>&nbsp;
<br>Here, we present the construction and early validation of a large naïve library from llamas and alpacas. Characterization data suggests that this library, the AbNanoTM VHH Naïve Library, may be well-suited for rapid discovery of VHH domains binding to therapeutic targets with varying levels of affinity. In this study we show how the AbNano VHH Naïve Library can be used to identify target specific VHHs to PD-L1, including live cell binding by flow cytometry, while reducing the time and resources necessary to do so.</div>

Target

PCDH19 mosaic mice help scientists decipher the neural underpinnings of developmental and epileptic encephalopathy 9.

Understanding Neuronal Network Activity in Developmental and Epileptic Encephalopathy 9

Developmental and epileptic encephalopathy 9 is a neurodevelopmental disorder caused by a mutation in <i>PCDH19</i>. Because disease only occurs in heterozygous <i>PCDH19</i> individuals, scientists developed a mosaic mouse model where some cells express PCDH19 and others do not. This model helped them understand that PCDH19-negative neurons are hyperexcitable in a DEE9 mouse model and how the brain responds as a result.