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Goat anti-Human IgA Cross-Adsorbed Antibody FITC Conjugated
Bethyl Laboratories
Catalog #
Target:
Human IgA
Reactivity:
Human
Applications:
ELISA,
Flow Cyt,
ICC,
IF,
IHC,
WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Biotin,
DyLight® 488,
DyLight® 550,
DyLight® 594,
DyLight® 650,
FITC,
HRP,
Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.5 mg)
Biotin (0.5 mg)
DyLight® 488 (0.5 mg)
DyLight® 550 (0.5 mg)
DyLight® 594 (0.5 mg)
DyLight® 650 (0.5 mg)
FITC (0.5 mg)
HRP (0.5 mg)
$106.00
$174.00
Product Details
Specifications
Verified Reactivity
Human
Antigen Species
Human
Minimal Reactivity
Mouse, Rat
Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change
Production Procedures
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgA was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgA was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to biotin.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgA was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgA was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgA was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgA was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgA was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgA was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgA was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to biotin.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgA was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgA was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgA was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgA was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgA was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species. Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human IgA was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgA. Cross reactivity with IgM and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgA was detected. This antibody may cross react with IgA from other species.
Country of Origin
USA
Additional Product Information
IgA is an immunoglobulin that is typically dimeric and held together by a J-chain protein. IgA is typically found near the mucous membranes of the body, including the gut, respiratory system, eyes, and colostrum. Also exists in a secretory form (in mucosal regions) vs. serum IgA, which is found in serum.
Applications
Not all listed applications have been specifically tested by our laboratory.
DyLight® 488 is excited at 493 (in PBS) and emits at 518 (in PBS).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 550 is excited at 562 (in PBS) and emits at 576 (in PBS). DyLight® 550 replaces DyLight® 549.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 594 is excited at 593 (in PBS) and emits at 618 (in PBS).
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
DyLight® 650 is excited at 652 (in PBS) and emits at 672 (in PBS). DyLight® 650 replaces DyLight® 649.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.