Goat anti-Monkey IgG Heavy and Light Chain Antibody Cross-Adsorbed Affinity Purified
Product Details
Specifications
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Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with monkey IgG and with light chains common to other monkey immunoglobulins. Species detected are Rhesus, and Cynomolgus. Reaction to human IgG was less than 1%. No antibody was detected against non-immunoglobulin serum proteins. The antibody was adsorbed against human serum, isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with monkey IgG and with light chains common to other monkey immunoglobulins. Species detected are Rhesus, Baboon, Carb Green, Cyno, Chimp and African Green. Reaction to human IgG was less than 1%. No antibody was detected against non-immunoglobulin serum proteins.
Additional Product Information
Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.
Applications
Not all listed applications have been specifically tested by our laboratory.