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Goat anti-Pig IgG Heavy and Light Chain Cross-Adsorbed Antibody FITC Conjugated
Bethyl Laboratories
Catalog #
Target:
Pig IgG Heavy and Light Chain
Reactivity:
Pig
Applications:
ELISA
,Flow Cyt
,ICC
,IF
,IHC
,WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
FITC
,HRP
,Unconjugated
Purity:
Antigen Affinity Purified
Product Details
Specifications
Verified Reactivity
Pig
Antigen Species
Pig
Minimal Reactivity
Bovine, Chicken, Horse, Human, Mouse, Rabbit, Rat
Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
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Production Procedures
Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to pig IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with pig IgG and with light chains common to other pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, horse, human, mouse, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to pig IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with pig IgG and with light chains common to other pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, horse, human, mouse, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to pig IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with pig IgG and with light chains common to other pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, horse, human, mouse, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
Country of Origin
USA
Additional Product Information
Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.
Applications