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Sheep anti-Dog IgG2 Heavy Chain Antibody HRP Conjugated
Bethyl Laboratories
Catalog #
Target:
Dog IgG2 Heavy Chain
Reactivity:
Dog
Applications:
ELISA,
ICC,
IF,
IHC,
WB
Host:
Sheep
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
FITC,
HRP,
Unconjugated
Purity:
Antigen Affinity Purified
Product Details
Specifications
Verified Reactivity
Dog
Antigen Species
Dog
Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt,
2 years from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Request Formulation Change
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Request Formulation Change
Production Procedures
Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with dog IgG2. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross react with IgG2 from other species. Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with dog IgG2. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross react with IgG2 from other species. Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with dog IgG2. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross react with IgG2 from other species.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with dog IgG2. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross react with IgG2 from other species. Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with dog IgG2. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross react with IgG2 from other species. Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with dog IgG2. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross react with IgG2 from other species.
Country of Origin
USA
Additional Product Information
IgG2 is the second most prominent subclass of IgG and does not cross the placenta easily but does bind strongly to Fc receptors. However, the half-life of IgG3 molecules is much shorter than IgG1.
Applications
Not all listed applications have been specifically tested by our laboratory.